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    日期:2015年12月3日 浏览次数:5,016


    1、CD73-mediatedadenosine production promotes stem cell-like properties in mouse Tc17 cells

    Felipe Flores-Santibáñez... Daniela Sauma, Immunology | 09/02/2015

    ... T cells werestimulated with 1 μg/ml α-CD3 antibody(clone 145-2C11, eBioscience) in the presence or absence of Tc17polarizing conditions: 20 ng/ml IL-6 (R&D Systems), 2 ng/ml TGF-β (eBioscience) ...


    2、PD-1 Blockade CanRestore Functions of T-Cells in Epstein-Barr Virus-Positive Diffuse LargeB-Cell Lymphoma In Vitro

    Lina Quan ... YueLiu, PLOS ONE | 09/11/2015

    ... in 96-wellU-bottom plates with or without 10 μg/mL anti-PD-1 (J116) orimmunoglobulin G1 (IgG1) isotype control mAbs (eBioscience, San Diego, CA, USA). ... added phytohemagglutinin (PHA) at 2μg/mL, with or without 10 μg/mL anti-PD-1 (J116) or IgG1 isotype control mAbs (eBioscience).


    3、L-selectincontrols trafficking of chronic lymphocytic leukemia cells in lymph node highendothelial venules in vivo

    Fanny Lafouresse,... Jean-Philippe Girard, Blood, Sep 2015; 126: 1336 -1345.

    ...pretreated for30 minutes with a function blockinganti-L-selectin (CD62L) antibody (Ab) (6 mug/mL, DREG56; eBioscience) or with an isotype control.


    4、dNP2 is ablood–brain barrier-permeable peptide enabling ctCTLA-4 protein delivery toameliorate experimental autoimmune encephalomyelitis

    SanghoLim ... Je-Min Choi, Nature Communications | 09/15/2015

    ... The cells weresubdivided into various cell types by staining with 1/800 diluted anti-mouseCD4-PerCP-Cy5.5 (#45-0042) and anti-mouse CD19-PE-Cy7 (#25-0193) or anti-mouseF4/80 PerCP-Cy5.5 (#45-4,801), anti-mouse MHCII-PE (#12-5,322-81), anti-mouseCD11b-PE-Cy7 (#25-0112) and anti-mouse CD11c-APC (#17-0114) FACS antibodies.The antibodies were purchased from eBioscience. ... cells were further stained with1/800 diluted anti-human CD4-PE-Cy7 (#25-0049), anti-human CD19-APC (#17-0199),anti-human CD11b-PE-Cy7 (#25-0118) or anti-human CD11c-APC (#17-0116) FACSantibodies purchased from eBioscience. ... Primary antibody staining wasperformed overnight using 1/100 diluted anti-mouse CD4-FITC (eBioscience, #11-0041), ... The surface of theisolated lymphocytes was stained with 1/800 diluted anti-mouse CD4 PerCP-Cy5.5antibody (eBioscience, #45-0042). The lymphocytes were also stained with 1/400diluted anti-mouse IFN-γ-FITC (eBioscience, #13-7,311) and 1/100 diluted IL-17A-APCantibodies (eBioscience, #17-7,177) using theFixation/Permeabilization concentrate and diluent kit (eBioscience). ... each tissue after staining with1/800 diluted anti-mouse CD4-PerCP-Cy5.5 (#45-0042), anti-mouse CD62L-FITC(#11-0621) and anti-mouse CD44-PE (#12-0441) FACS antibodies purchased from eBioscience.


    5、TIR-domain-containingadapter-inducing interferon-β (TRIF) mediates immunological memory againstbacterial pathogens

    SaravanaKanagavelu ... Masayuki Fukata, Infection and Immunity | 09/08/2015

    ... Recombinant IL-17(3ng/ml) (eBioscience) was added for 60 minat 37oC. Peritoneal Mφs from WT ... Surface staining of CD3, CD4, CD44,CD62L, CD127 and Gr1 was performed according to the manufacturer's instructions(eBioscience, San Diego, CA).


    6、DC Maturation: ABrief Comparison between Three Different Processes

    Maria Toloudi,Ioannis Papasotiriou, Journal of Cancer Therapy | 2015, 6, 871-880

    ...and 500 ng/ml CD40L (Human CD154 (CD40 Ligand)Recombinant Protein, 14-8502, eBioscience). ... along with FITC-conjugated anti-CD14antibody (anti-human CD14 FITC, 11-0149-42, eBioscience) at room temperature ...


    7、PD-L1 (B7-H1)Expression and the Immune Tumor Microenvironment in Primary and MetastaticBreast Carcinomas

    AshleyCimino-Mathews, ... Leisha A. Emens, Human Pathology, Accepted Manuscript,online 21 Sep 2015

    eBioexcerpt not available.


    8、Phenotypic Tfhdevelopment promoted by CXCR5-controlled re-localization and IL-6 fromradiation-resistant cells

    Xin Chen ... HaiQi, Protein & Cell | 09/24/2015

    ... Stainingreagents included ... purified anti-PD-1 from eBioscience, ... and purified hamster IgG from eBioscience. ... To detect intracellular Bcl6, cellswere stained in the Foxp3/Transcription Factor Staining Buffer Set (eBioscience) after surface staining. ... Stainingreagents included eFluor450 anti-CD3 (eBioscience) and AlexaFluor 647 anti-IgD (eBioscience).


    9、IL-22 DefectDuring Streptococcus pneumoniae Infection Triggers Exacerbation of ChronicObstructive Pulmonary Disease

    Muriel Pichavant,... Philippe Gosset, EBioMedicine, In Press, Accepted Manuscript, Available online 26September 2015

    ... and incubatedwith PE-conjugated mAb against IL-22 (eBiosciences) ... lung cells wereseeded on 96-well plates coated or not with anti-CD3 Ab (eBiosciences).


    10、Conserved Motifswithin Hepatitis C Virus Envelope (E2) RNA and Protein Independently Inhibit TCell Activation

    Nirjal Bhattarai,... Jack T. Stapleton, PLOS Pathogens | 09/30/2015

    ...PBMCs (1×106 cells/ml) were stimulated with plate-bound anti-CD3 (100ng/ml,OKT3 clone, eBioscience) ...


    11、Ikaros mediates the DNAmethylation-independent silencing of MCJ/DNAJC15 gene expression in macrophages

    Nicolás Navasa ...Juan Anguita, Scientific Reports | 09/30/2015

    ... Upon incubation with100 ng/mL of IFNγ (eBioScience, San Diego, CA) for 3days, ...


    12、T cell receptorreversed polarity recognition of a self-antigen major histocompatibilitycomplex

    Dennis X Beringer... Jamie Rossjohn, Nature Immunology | 10/05/2015

    ... Respondercells were labeled with 1 μM CFSE and stimulated with 1μg/ml anti-CD3 (clone UCHT1, eBiosciences, San Diego, CA), ... Antibodies to following markers (clonenames are in parentheses) were used: PD1 (J116), granzyme B (GB11), FAS (DX2),GITR (ebioAITR), CD45RO (UCHL1), CD25 (M-A251), IgG2a (eBB2a) and IgG1 (P3)were from eBiosciences (San Diego, CA).


    13、MFG-E8 IsCritical for Embryonic Stem Cell-Mediated T Cell Immunomodulation

    Yuan Tan ...Lisheng Wang, Stem Cell Reports, In Press, Corrected Proof, Availableonline 8 October 2015

    ...followed by stimulation with plate-coated anti-CD3 and anti-CD28 (eBioscience, catalog no. 16-0031-85and 16-0281-85) for 48 hr. Cells were then stained with antibodiesagainst CD4, CD8, and surface activation markers (CD25, CD44, and CD69) (eBioscience, catalog no. 15-0041-82, 11-0081-81,48-0253-82, 25-0691-81, and 47-0441-82, respectively).


    14、Modulation ofDendritic Cell Activation and Subsequent Th1 Cell Polarization by Lidocaine

    Young-Tae Jeon,... Yeonseok Chung, PLOS ONE | 10/07/2015

    ... (all fromBiolegend except IL–13 from eBioscience). ... For Th2 differentiation,IL–2 (10 ng/ml) (eBioscience), ... in the presence of Brefeldin A andMonesin (Both from eBioscience) for 4 h. ... cells were fixed withfixation buffer (eBioscience) for 30 min at 4°C and washed withpermeabilization buffer (eBioscience). For Foxp3 staining, a Foxp3 stainingkit (eBioscience) ...


    15、Inflammation-associated alterations tothe intestinal microbiota reduce colonization resistance against non-typhoidalSalmonella during concurrent malaria parasite infection

    Jason P. Mooney... Renée M. Tsolis, Scientific Reports | 10/05/2015

    ... Next, cells were blocked with 4 μL anti-CD16/32 antibody (clone 93; eBioscience) ...


    16、Direct tumor recognition by a human CD4+T-cell subset potently mediates tumor growth inhibition and orchestratesanti-tumor immune responses [open access]

    Junko Matsuzaki... Kunle Odunsi, Scientific Reports | 10/08/2015

    ... blockade ofCD40-CD40L and CD27-CD70 using antibodies (anti-CD40 antibody: clone 5C3 from eBioscience, and anti-CD27 antibody: clone LG.3A10fromeBioscience) ... cancer cells were treated with20 μg/ml Fas-ligand antibodies (eBiosciences) ... and anti-IL-2 antibody (eBioscience) ... co-cultured with 2 × 105TR-CD4 in the presence or absence of 10 μg/ml anti-IFN-γ (eBiosciences) ... 10 μg/mlanti-TNF-α mAb (eBiosciences) ... kits for IL-2, IFN-γ and TNF-α wereobtained fromeBioscience ... and 5 μg/ml anti-CD3 mAb (OKT3; eBioscience)-coated plate ... Transduction efficacywas measured for TR-CD4-chTCR by staining with anti-human TCR Vβ5.1 mAb (eBioscience).